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KMID : 0368420150580010017
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Journal of Plant Biology
2015 Volume.58 No. 1 p.17 ~ p.25
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GmREM1.1 and GmREM2.1, which encode the remorin proteins in soybean, have distinct roles during root nodule development
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Son Seung-Min
Oh Chang-Jae
Bae Ju-Hee
Lee Hyoung-Seok
An Chung-Sun
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Abstract
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Remorin is involved in various biotic and abiotic stresses, including host-microbe interactions. To elucidate its roles during root nodule development, we isolated and characterized five remorin genes in soybean. The genes belonging to groups 1 and 2 were both expressed during root nodule development but with somewhat different temporal patterns. Using a biochemical assay, we showed that the homo-oligomerization of GmREM1.1 was mediated by the coiled-coil region in the C-terminal domain in combination with either the N-terminal or C-terminal anchor peptides (RemCA). Plasma membrane targeting was also sufficiently mediated by RemCA from GmREM1s but not from GmREM2.1. In addition, GmREM1.1 was highly expressed in the nodule primordia and inner cortex region of root nodules, whereas GmREM2.1 transcription was mainly detected in the infected cells. Moreover, RNAi-GmREM2.1 hairy roots showed significantly reduced nodule formation, but RNAi-GmREM1.1 had little effect on nodule formation. Taken together, our results suggest that GmREM1.1 and GmREM2.1, with different molecular features, function distinctively during root nodule development.
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KEYWORD
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Expression pattern, Nodule development, Oligomer, RemCA, Remorin, Soybean
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